Lack of Artemisinin Resistance in Plasmodium falciparum in Uganda Based on Parasitological and Molecular Assays

Roland A. Cooper, Melissa D. Conrad, Quentin D. Watson, Stephanie J. Huezo, Harriet Ninsiima, Patrick Tumwebaze, Samuel L. Nsobya, Philip J. Rosenthal

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated markers of artemisinin resistance in Plasmodium falciparum isolated in Kampala in 2014. By standard in vitro assays, all isolates were highly sensitive to dihydroartemisinin (DHA). By the ring-stage survival assay, after a 6-h DHA pulse, parasitemia was undetectable in 40 of 43 cultures at 72 h. Two of 53 isolates had nonsynonymous K13-propeller gene polymorphisms but did not have the mutations associated with resistance in Asia. Thus, we did not see evidence for artemisinin resistance in Uganda.

Original languageAmerican English
Pages (from-to)5061-5064
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume59
Issue number8
DOIs
StatePublished - Aug 1 2015

Funding

FundersFunder number
National Institutes of HealthT37MD003407, D43TW007375, AI1R01AI075045
National Institute of Allergy and Infectious DiseasesU19AI089674

    ASJC Scopus Subject Areas

    • Pharmacology
    • Pharmacology (medical)
    • Infectious Diseases

    Keywords

    • Artemisinin Resistance
    • Plasmodium falciparum

    Disciplines

    • Medicinal Chemistry and Pharmaceutics
    • Parasitic Diseases
    • Pharmacology, Toxicology and Environmental Health

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