Lack of resistance of Plasmodium falciparum to dihydroartemisinin in Uganda based on parasitogolgical and molecular assays

Roland A. Cooper; Melissa D. Conrad; Quentin D. Watson; Stephanie J. Huezo; Harriet Ninsiima; Patrick Tumwebaze; Samuel L. Nsobya; Philip J. Rosenthal

Lack of resistance of Plasmodium falciparum to dihydroartemisinin in Uganda based on parasitogolgical and molecular assays

Roland A. Cooper
, Melissa D. Conrad
, Quentin D. Watson
, Stephanie J. Huezo
, Harriet Ninsiima
, Patrick Tumwebaze
, Samuel L. Nsobya
, Philip J. Rosenthal

Department of Natural Sciences and Mathematics

Research output: Contribution to conference › Presentation

Abstract

Artemisinin-‐based combination therapy is now standard treatment for falciparum malaria. However, this regimen is threatened by resistance to artemisinins, manifest as delayed clearance of parasitemia after therapy, in southeast Asia.
Artemisinin resistance in southeast Asia is associated with increased parasitemias in culture, compared to those in sensi0ve parasites, 72 hours a=er a 6 hour pulse with 700 nM dihydroartemisinin (DHA), and with propeller domain polymorphisms in the Plasmodium falciparum kelch (K13; PF3D7_1343700) gene
Given that artemether/lumefantrine has been adopted as standard therapy for malaria within the last decade in Uganda, we characterized artemisinin sensiBvity in fresh P. falciparum isolates from Kampala using ex vivo ring-‐stage survival and IC50 assays. We also assessed the K13 gene for polymorphisms.

Original language	American English
State	Published - Oct 25 2015
Event	American Society of Tropical Medicine and Hygiene Annual Conference - Philadelphia, United States Duration: Oct 25 2015 → Oct 29 2015 Conference number: 64th

Conference

Conference	American Society of Tropical Medicine and Hygiene Annual Conference
Abbreviated title	ASTMH2015
Country/Territory	United States
City	Philadelphia
Period	10/25/15 → 10/29/15

Keywords

malaria
artemisinin

Disciplines

Life Sciences
Medicinal Chemistry and Pharmaceutics
Parasitic Diseases
Pharmacology, Toxicology and Environmental Health

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title = "Lack of resistance of Plasmodium falciparum to dihydroartemisinin in Uganda based on parasitogolgical and molecular assays",

abstract = "Artemisinin-‐based combination therapy is now standard treatment for falciparum malaria. However, this regimen is threatened by resistance to artemisinins, manifest as delayed clearance of parasitemia after therapy, in southeast Asia. Artemisinin resistance in southeast Asia is associated with increased parasitemias in culture, compared to those in sensi0ve parasites, 72 hours a=er a 6 hour pulse with 700 nM dihydroartemisinin (DHA), and with propeller domain polymorphisms in the Plasmodium falciparum kelch (K13; PF3D7\_1343700) gene Given that artemether/lumefantrine has been adopted as standard therapy for malaria within the last decade in Uganda, we characterized artemisinin sensiBvity in fresh P. falciparum isolates from Kampala using ex vivo ring-‐stage survival and IC50 assays. We also assessed the K13 gene for polymorphisms. ",

keywords = "malaria, artemisinin",

author = "Cooper, \{Roland A.\} and Conrad, \{Melissa D.\} and Watson, \{Quentin D.\} and Huezo, \{Stephanie J.\} and Harriet Ninsiima and Patrick Tumwebaze and Nsobya, \{Samuel L.\} and Rosenthal, \{Philip J.\}",

year = "2015",

month = oct,

day = "25",

language = "American English",

note = "American Society of Tropical Medicine and Hygiene Annual Conference, ASTMH2015 ; Conference date: 25-10-2015 Through 29-10-2015",

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TY - CONF

T1 - Lack of resistance of Plasmodium falciparum to dihydroartemisinin in Uganda based on parasitogolgical and molecular assays

AU - Cooper, Roland A.

AU - Conrad, Melissa D.

AU - Watson, Quentin D.

AU - Huezo, Stephanie J.

AU - Ninsiima, Harriet

AU - Tumwebaze, Patrick

AU - Nsobya, Samuel L.

AU - Rosenthal, Philip J.

N1 - Conference code: 64th

PY - 2015/10/25

Y1 - 2015/10/25

N2 - Artemisinin-‐based combination therapy is now standard treatment for falciparum malaria. However, this regimen is threatened by resistance to artemisinins, manifest as delayed clearance of parasitemia after therapy, in southeast Asia. Artemisinin resistance in southeast Asia is associated with increased parasitemias in culture, compared to those in sensi0ve parasites, 72 hours a=er a 6 hour pulse with 700 nM dihydroartemisinin (DHA), and with propeller domain polymorphisms in the Plasmodium falciparum kelch (K13; PF3D7_1343700) gene Given that artemether/lumefantrine has been adopted as standard therapy for malaria within the last decade in Uganda, we characterized artemisinin sensiBvity in fresh P. falciparum isolates from Kampala using ex vivo ring-‐stage survival and IC50 assays. We also assessed the K13 gene for polymorphisms.

AB - Artemisinin-‐based combination therapy is now standard treatment for falciparum malaria. However, this regimen is threatened by resistance to artemisinins, manifest as delayed clearance of parasitemia after therapy, in southeast Asia. Artemisinin resistance in southeast Asia is associated with increased parasitemias in culture, compared to those in sensi0ve parasites, 72 hours a=er a 6 hour pulse with 700 nM dihydroartemisinin (DHA), and with propeller domain polymorphisms in the Plasmodium falciparum kelch (K13; PF3D7_1343700) gene Given that artemether/lumefantrine has been adopted as standard therapy for malaria within the last decade in Uganda, we characterized artemisinin sensiBvity in fresh P. falciparum isolates from Kampala using ex vivo ring-‐stage survival and IC50 assays. We also assessed the K13 gene for polymorphisms.

KW - malaria

KW - artemisinin

UR - https://scholar.dominican.edu/all-faculty/89

M3 - Presentation

T2 - American Society of Tropical Medicine and Hygiene Annual Conference

Y2 - 25 October 2015 through 29 October 2015

ER -

Lack of resistance of Plasmodium falciparum to dihydroartemisinin in Uganda based on parasitogolgical and molecular assays

Abstract

Conference

Keywords

Disciplines

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